AffiELISA® Multiplex ELISA Kit For Porcine Cytokine Array (4-plex)

Specifications

Description

Product Overview The Multiplex Porcine Cytokine – High Sensitivity (4-plex) is a fully quantitative, ELISA-based chemiluminescent assay allowing concurrent measurement of cytokines in serum or plasma samples. A kit contains a 96-well plate, with each well featuring the 4-plex along with a positive control with all the other reagents necessary to perform the test. Our high-quality reagents help ensure the accuracy of your results. Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 4.83 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample. Assay Ranges Analytes Calibrator Range Units LLD IL-1β 1550 - 2.13 pg/mL 2.1 IL-6 1800 - 2.47 pg/mL 2.5 IL-8 2100 - 2.88 pg/mL 2.9 TNFα 1600 - 2.19 pg/mL 1.9 Analytes Calibrator Range Units LLD Analytes Calibrator Range Units LLD Analytes Calibrator Range Units LLD IL-1β 1550 - 2.13 pg/mL 2.1 IL-6 1800 - 2.47 pg/mL 2.5 IL-8 2100 - 2.88 pg/mL 2.9 TNFα 1600 - 2.19 pg/mL 1.9 IL-1β 1550 - 2.13 pg/mL 2.1 IL-1β 1550 - 2.13 pg/mL 2.1 IL-6 1800 - 2.47 pg/mL 2.5 IL-6 1800 - 2.47 pg/mL 2.5 IL-8 2100 - 2.88 pg/mL 2.9 IL-8 2100 - 2.88 pg/mL 2.9 TNFα 1600 - 2.19 pg/mL 1.9 TNFα 1600 - 2.19 pg/mL 1.9 Kit Components Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization. Reagents Include: Calibrator: Lyophilized, recombinant antigens in a buffered protein base Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with blockers and preservatives Streptavidin HRP (1X) : Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase Wash Buffer (20X) : Liquid, 50 mL/vial of a concentrated solution of buffered surfactant Plate Seals (3) : Adhesive strips