AffiELISA® Multiplex ELISA Kit For Human Micronutrient Array (7-plex)

Specifications

Description

Product Overview The Multiplex Human Micronutrient Array (7-plex) is a fully quantitative chemiluminescent assay allowing concurrent measurement of biomarkers used in nutritional assessment in serum or heparin plasma samples. Analytes CRP, Ferritin, HRP2, sTfR, and Thyroglobulin (Tg) are sandwich ELISAs while AGP and RBP4 are competitive ELISAs. This product is alternatively known in previous publications as Multiplex Micronutrient Assessment Tool (MMAT) . A Human Micronutrient Assay kit contains a 96-well plate, with each well featuring the 7-plex along with reagents necessary to perform the test. Our high quality reagents help ensure the accuracy of your results. Using just 5 µl of sample per well, up to 80 wells can be tested for all seven markers in the panel in 3.5 hours. Multiplex Arrays provide scientists with an easy-to-use and cost effective means of generating a biomarker profile for each sample. Assay Ranges Analytes Assay Types Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection AGP Competitive 0.36-0.0005 (g/L) 0.25 (g/L) 0.0019 (g/L) 0.00096 (g/L) CRP Sandwich 1-0.0014 (mg/L) 0.7 (mg/L) 0.0018 (mg/L) 0.00089 (mg/L) Ferritin Sandwich 23-0.032 (µg/L) 16.17 (µg/L) 0.041 (µg/L) 0.021 (µg/L) HRP2 Sandwich 1.9-0.0026 (µg/L) 1.33 (µg/L) 0.0034 (µg/L) 0.0017 (µg/L) RBP4 Competitive 1.01-0.0014 (µmol/L) 0.71 (µmol/L) 0.0054 (µmol/L) 0.0027 (µmol/L) sTFR Sandwich 4-0.0055 (mg/L) 2.8 (mg/L) 0.0071 (mg/L) 0.0036 (mg/L) Thyroglobulin Sandwich 15.1-0.021 (µg/L) 10.57 (µg/L) 0.027 (µg/L) 0.013 (µg/L) Analytes Assay Types Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analytes Assay Types Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analytes Assay Types Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection AGP Competitive 0.36-0.0005 (g/L) 0.25 (g/L) 0.0019 (g/L) 0.00096 (g/L) CRP Sandwich 1-0.0014 (mg/L) 0.7 (mg/L) 0.0018 (mg/L) 0.00089 (mg/L) Ferritin Sandwich 23-0.032 (µg/L) 16.17 (µg/L) 0.041 (µg/L) 0.021 (µg/L) HRP2 Sandwich 1.9-0.0026 (µg/L) 1.33 (µg/L) 0.0034 (µg/L) 0.0017 (µg/L) RBP4 Competitive 1.01-0.0014 (µmol/L) 0.71 (µmol/L) 0.0054 (µmol/L) 0.0027 (µmol/L) sTFR Sandwich 4-0.0055 (mg/L) 2.8 (mg/L) 0.0071 (mg/L) 0.0036 (mg/L) Thyroglobulin Sandwich 15.1-0.021 (µg/L) 10.57 (µg/L) 0.027 (µg/L) 0.013 (µg/L) AGP Competitive 0.36-0.0005 (g/L) 0.25 (g/L) 0.0019 (g/L) 0.00096 (g/L) AGP Competitive 0.36-0.0005 (g/L) 0.25 (g/L) 0.0019 (g/L) 0.00096 (g/L) CRP Sandwich 1-0.0014 (mg/L) 0.7 (mg/L) 0.0018 (mg/L) 0.00089 (mg/L) CRP Sandwich 1-0.0014 (mg/L) 0.7 (mg/L) 0.0018 (mg/L) 0.00089 (mg/L) Ferritin Sandwich 23-0.032 (µg/L) 16.17 (µg/L) 0.041 (µg/L) 0.021 (µg/L) Ferritin Sandwich 23-0.032 (µg/L) 16.17 (µg/L) 0.041 (µg/L) 0.021 (µg/L) HRP2 Sandwich 1.9-0.0026 (µg/L) 1.33 (µg/L) 0.0034 (µg/L) 0.0017 (µg/L) HRP2 Sandwich 1.9-0.0026 (µg/L) 1.33 (µg/L) 0.0034 (µg/L) 0.0017 (µg/L) RBP4 Competitive 1.01-0.0014 (µmol/L) 0.71 (µmol/L) 0.0054 (µmol/L) 0.0027 (µmol/L) RBP4 Competitive 1.01-0.0014 (µmol/L) 0.71 (µmol/L) 0.0054 (µmol/L) 0.0027 (µmol/L) sTFR Sandwich 4-0.0055 (mg/L) 2.8 (mg/L) 0.0071 (mg/L) 0.0036 (mg/L) sTFR Sandwich 4-0.0055 (mg/L) 2.8 (mg/L) 0.0071 (mg/L) 0.0036 (mg/L) Thyroglobulin Sandwich 15.1-0.021 (µg/L) 10.57 (µg/L) 0.027 (µg/L) 0.013 (µg/L) Thyroglobulin Sandwich 15.1-0.021 (µg/L) 10.57 (µg/L) 0.027 (µg/L) 0.013 (µg/L) Kit Components Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization. Reagents Include: Calibrator: Lyophilized, recombinant antigens in a buffered protein base Calibrator Additive: Purified ferritin antigen in a buffered protein base Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives Competitor: Lyophilized, biotinylated competitive antigen Substrate A: Liquid, 3.5 mL/vial Substrate B: Liquid, 3.5 mL/vial Sample Diluent (2X) : Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives Streptavidin HRP (1X) : Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase Wash Buffer (20X) : Liquid, 50 mL/vial of a concentrated solution of buffered surfactant Plate Seals (3) : Adhesive strips