AffiELISA® Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex)

Specifications

Description

Product Overview The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Using only 25 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 4.83 hours. The Multiplex ELISA Kit For Human Cytokine Release Syndrome (16-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results. Assay Ranges Analytes Calibrator Range Units LLD GMCSF 6,000-10.70 pg/mL 5.35 IFNα 400-0.72 pg/mL 0.36 IFNγ 99-0.18 pg/mL 0.09 IL-1β 4,075-7.27 pg/mL 3.64 IL-1RA 7,000-12.48 pg/mL 6.24 IL-2 64-0.11 pg/mL 0.06 IL-2Rα 16,000-28.5 pg/mL 14.25 IL-6 333-0.59 pg/mL 0.3 IL-6R 1,250-2.23 pg/mL 1.11 IL-8 2,000-3.6 pg/mL 1.8 IL-10 2,680-4.78 pg/mL 2.39 IL-12p70 3,000-5.35 pg/mL 2.68 IL-13 333-0.59 pg/mL 0.3 MCP-1 2,000-3.6 pg/mL 1.8 MIP-1α 2,000-3.57 pg/mL 1.78 TNFα 661-1.18 pg/mL 0.59 Analytes Calibrator Range Units LLD Analytes Calibrator Range Units LLD Analytes Calibrator Range Units LLD GMCSF 6,000-10.70 pg/mL 5.35 IFNα 400-0.72 pg/mL 0.36 IFNγ 99-0.18 pg/mL 0.09 IL-1β 4,075-7.27 pg/mL 3.64 IL-1RA 7,000-12.48 pg/mL 6.24 IL-2 64-0.11 pg/mL 0.06 IL-2Rα 16,000-28.5 pg/mL 14.25 IL-6 333-0.59 pg/mL 0.3 IL-6R 1,250-2.23 pg/mL 1.11 IL-8 2,000-3.6 pg/mL 1.8 IL-10 2,680-4.78 pg/mL 2.39 IL-12p70 3,000-5.35 pg/mL 2.68 IL-13 333-0.59 pg/mL 0.3 MCP-1 2,000-3.6 pg/mL 1.8 MIP-1α 2,000-3.57 pg/mL 1.78 TNFα 661-1.18 pg/mL 0.59 GMCSF 6,000-10.70 pg/mL 5.35 GMCSF 6,000-10.70 pg/mL 5.35 IFNα 400-0.72 pg/mL 0.36 IFNα 400-0.72 pg/mL 0.36 IFNγ 99-0.18 pg/mL 0.09 IFNγ 99-0.18 pg/mL 0.09 IL-1β 4,075-7.27 pg/mL 3.64 IL-1β 4,075-7.27 pg/mL 3.64 IL-1RA 7,000-12.48 pg/mL 6.24 IL-1RA 7,000-12.48 pg/mL 6.24 IL-2 64-0.11 pg/mL 0.06 IL-2 64-0.11 pg/mL 0.06 IL-2Rα 16,000-28.5 pg/mL 14.25 IL-2Rα 16,000-28.5 pg/mL 14.25 IL-6 333-0.59 pg/mL 0.3 IL-6 333-0.59 pg/mL 0.3 IL-6R 1,250-2.23 pg/mL 1.11 IL-6R 1,250-2.23 pg/mL 1.11 IL-8 2,000-3.6 pg/mL 1.8 IL-8 2,000-3.6 pg/mL 1.8 IL-10 2,680-4.78 pg/mL 2.39 IL-10 2,680-4.78 pg/mL 2.39 IL-12p70 3,000-5.35 pg/mL 2.68 IL-12p70 3,000-5.35 pg/mL 2.68 IL-13 333-0.59 pg/mL 0.3 IL-13 333-0.59 pg/mL 0.3 MCP-1 2,000-3.6 pg/mL 1.8 MCP-1 2,000-3.6 pg/mL 1.8 MIP-1α 2,000-3.57 pg/mL 1.78 MIP-1α 2,000-3.57 pg/mL 1.78 TNFα 661-1.18 pg/mL 0.59 TNFα 661-1.18 pg/mL 0.59 Kit Components Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. Reagents include: Wash Buffer Concentrate (20X) : Liquid, 50 mL/vial of a concentrated solution of buffered surfactant Sample Diluent: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered solution with preservatives Calibrator: Lyophilized, recombinant antigens in a buffered protein base Streptavidin-HRP (1X) : Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide Substrate B+: Liquid, 3 mL/vial of stabilized signal enhancer Plate Seals (3) : Adhesive strips