AffiELISA® Multiplex ELISA Kit For Human Cytokine Panel 2 (4-Plex)

Specifications

Description

Product Overview The Multiplex ELISA Kit For Human Cytokine Panel 2 (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IL-1α, IL-4, IL-6, TNFα) . Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results. Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 2.25 hours. Multiplex ELISA Kit For Human Cytokine Panel 2 (4-Plex) provides scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample. Assay Ranges Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection IL-1α Sandwich 4000 -5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.43 (pg/mL) IL-4 Sandwich 1676 – 2.30 (pg/mL) 1173 (pg/mL) 3.0 (pg/mL) 1.79 (pg/mL) IL-6 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 3.47 (pg/mL) TNFα Sandwich 6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection IL-1α Sandwich 4000 -5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.43 (pg/mL) IL-4 Sandwich 1676 – 2.30 (pg/mL) 1173 (pg/mL) 3.0 (pg/mL) 1.79 (pg/mL) IL-6 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 3.47 (pg/mL) TNFα Sandwich 6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) IL-1α Sandwich 4000 -5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.43 (pg/mL) IL-1α Sandwich 4000 -5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.43 (pg/mL) IL-4 Sandwich 1676 – 2.30 (pg/mL) 1173 (pg/mL) 3.0 (pg/mL) 1.79 (pg/mL) IL-4 Sandwich 1676 – 2.30 (pg/mL) 1173 (pg/mL) 3.0 (pg/mL) 1.79 (pg/mL) IL-6 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 3.47 (pg/mL) IL-6 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 3.47 (pg/mL) TNFα Sandwich 6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) TNFα Sandwich 6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) Kit Components Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing. Reagents Include: Calibrator: Lyophilized, recombinant antigens in a buffered protein base Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives Sample Diluent: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives Streptavidin HRP (1X) : Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase Substrate A: Liquid, 3mL/vial of stabilized hydrogen peroxide Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer Wash Buffer Concentrate (20X) : Liquid, 50 mL/vial of a concentrated solution of buffered surfactant Plate Seals (3) : Adhesive strips