AffiELISA® Multiplex ELISA Kit For Human Cytokine HS Screen (15-Plex)

Specifications

Description

Product Overview The Multiplex Human Cytokine HS Screen (15-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 15 biomarkers or analytes. Using only 25 µl of sample per well, up to 80 samples can be assayed for all 15 markers in the panel within 4.83 hours. The Multiplex Human Cytokine HS Screen (15-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 15 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results. Assay Ranges Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection IFNγ Sandwich 99 – 0.14 (pg/mL) 69.3 (pg/mL) 0.18 (pg/mL) 0.10 (pg/mL) IL-1α Sandwich 1333 – 1.83 (pg/mL) 933 (pg/mL) 2.38 (pg/mL) 1.40 (pg/mL) IL-1β Sandwich 4075 – 5.59 (pg/mL) 2852 (pg/mL) 7.27 (pg/mL) 2.14 (pg/mL) IL-2 Sandwich 64 – 0.09 (pg/mL) 44.8 (pg/mL) 0.11 (pg/mL) 0.09 (pg/mL) IL-4 Sandwich 21 – 0.03 (pg/mL) 14.7 (pg/mL) 0.04 (pg/mL) 0.02 (pg/mL) IL-5 Sandwich 333 – 0.46 (pg/mL) 233 (pg/mL) 0.59 (pg/mL) 0.20 (pg/mL) IL-6 Sandwich 333 – 0.46 (pg/mL) 233 (pg/mL) 0.59 (pg/mL) 0.20 (pg/mL) IL-10 Sandwich 2680 – 3.68 (pg/mL) 1876 (pg/mL) 4.78 (pg/mL) 2.98 (pg/mL) IL-12p70 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.35 (pg/mL) 3.20 (pg/mL) IL-13 Sandwich 333 – 0.46 (pg/mL) 233 (pg/mL) 0.59 (pg/mL) 0.23 (pg/mL) IL-15 Sandwich 3578 – 4.91 (pg/mL) 2504 (pg/mL) 6.38 (pg/mL) 1.54 (pg/mL) IL-17A Sandwich 5589 – 7.67 (pg/mL) 3912 (pg/mL) 9.97 (pg/mL) 6.21 (pg/mL) IL-23 Sandwich 10000 – 13.72 (pg/mL) 6230 (pg/mL) 57.3 (pg/mL) 3.48 (pg/mL) TNFα Sandwich 661 – 0.91 (pg/mL) 462 (pg/mL) 1.18 (pg/mL) 0.10 (pg/mL) TNFβ Sandwich 444 – 0.61 (pg/mL) 310 (pg/mL) 0.79 (pg/mL) 0.57 (pg/mL) Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection IFNγ Sandwich 99 – 0.14 (pg/mL) 69.3 (pg/mL) 0.18 (pg/mL) 0.10 (pg/mL) IL-1α Sandwich 1333 – 1.83 (pg/mL) 933 (pg/mL) 2.38 (pg/mL) 1.40 (pg/mL) IL-1β Sandwich 4075 – 5.59 (pg/mL) 2852 (pg/mL) 7.27 (pg/mL) 2.14 (pg/mL) IL-2 Sandwich 64 – 0.09 (pg/mL) 44.8 (pg/mL) 0.11 (pg/mL) 0.09 (pg/mL) IL-4 Sandwich 21 – 0.03 (pg/mL) 14.7 (pg/mL) 0.04 (pg/mL) 0.02 (pg/mL) IL-5 Sandwich 333 – 0.46 (pg/mL) 233 (pg/mL) 0.59 (pg/mL) 0.20 (pg/mL) IL-6 Sandwich 333 – 0.46 (pg/mL) 233 (pg/mL) 0.59 (pg/mL) 0.20 (pg/mL) IL-10 Sandwich 2680 – 3.68 (pg/mL) 1876 (pg/mL) 4.78 (pg/mL) 2.98 (pg/mL) IL-12p70 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.35 (pg/mL) 3.20 (pg/mL) IL-13 Sandwich 333 – 0.46 (pg/mL) 233 (pg/mL) 0.59 (pg/mL) 0.23 (pg/mL) IL-15 Sandwich 3578 – 4.91 (pg/mL) 2504 (pg/mL) 6.38 (pg/mL) 1.54 (pg/mL) IL-17A Sandwich 5589 – 7.67 (pg/mL) 3912 (pg/mL) 9.97 (pg/mL) 6.21 (pg/mL) IL-23 Sandwich 10000 – 13.72 (pg/mL) 6230 (pg/mL) 57.3 (pg/mL) 3.48 (pg/mL) TNFα Sandwich 661 – 0.91 (pg/mL) 462 (pg/mL) 1.18 (pg/mL) 0.10 (pg/mL) TNFβ Sandwich 444 – 0.61 (pg/mL) 310 (pg/mL) 0.79 (pg/mL) 0.57 (pg/mL) IFNγ Sandwich 99 – 0.14 (pg/mL) 69.3 (pg/mL) 0.18 (pg/mL) 0.10 (pg/mL) IFNγ Sandwich 99 – 0.14 (pg/mL) 69.3 (pg/mL) 0.18 (pg/mL) 0.10 (pg/mL) IL-1α Sandwich 1333 – 1.83 (pg/mL) 933 (pg/mL) 2.38 (pg/mL) 1.40 (pg/mL) IL-1α Sandwich 1333 – 1.83 (pg/mL) 933 (pg/mL) 2.38 (pg/mL) 1.40 (pg/mL) IL-1β Sandwich 4075 – 5.59 (pg/mL) 2852 (pg/mL) 7.27 (pg/mL) 2.14 (pg/mL) IL-1β Sandwich 4075 – 5.59 (pg/mL) 2852 (pg/mL) 7.27 (pg/mL) 2.14 (pg/mL) IL-2 Sandwich 64 – 0.09 (pg/mL) 44.8 (pg/mL) 0.11 (pg/mL) 0.09 (pg/mL) IL-2 Sandwich 64 – 0.09 (pg/mL) 44.8 (pg/mL) 0.11 (pg/mL) 0.09 (pg/mL) IL-4 Sandwich 21 – 0.03 (pg/mL) 14.7 (pg/mL) 0.04 (pg/mL) 0.02 (pg/mL) IL-4 Sandwich 21 – 0.03 (pg/mL) 14.7 (pg/mL) 0.04 (pg/mL) 0.02 (pg/mL) IL-5 Sandwich 333 – 0.46 (pg/mL) 233 (pg/mL) 0.59 (pg/mL) 0.20 (pg/mL) IL-5 Sandwich 333 – 0.46 (pg/mL) 233 (pg/mL) 0.59 (pg/mL) 0.20 (pg/mL) IL-6 Sandwich 333 – 0.46 (pg/mL) 233 (pg/mL) 0.59 (pg/mL) 0.20 (pg/mL) IL-6 Sandwich 333 – 0.46 (pg/mL) 233 (pg/mL) 0.59 (pg/mL) 0.20 (pg/mL) IL-10 Sandwich 2680 – 3.68 (pg/mL) 1876 (pg/mL) 4.78 (pg/mL) 2.98 (pg/mL) IL-10 Sandwich 2680 – 3.68 (pg/mL) 1876 (pg/mL) 4.78 (pg/mL) 2.98 (pg/mL) IL-12p70 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.35 (pg/mL) 3.20 (pg/mL) IL-12p70 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.35 (pg/mL) 3.20 (pg/mL) IL-13 Sandwich 333 – 0.46 (pg/mL) 233 (pg/mL) 0.59 (pg/mL) 0.23 (pg/mL) IL-13 Sandwich 333 – 0.46 (pg/mL) 233 (pg/mL) 0.59 (pg/mL) 0.23 (pg/mL) IL-15 Sandwich 3578 – 4.91 (pg/mL) 2504 (pg/mL) 6.38 (pg/mL) 1.54 (pg/mL) IL-15 Sandwich 3578 – 4.91 (pg/mL) 2504 (pg/mL) 6.38 (pg/mL) 1.54 (pg/mL) IL-17A Sandwich 5589 – 7.67 (pg/mL) 3912 (pg/mL) 9.97 (pg/mL) 6.21 (pg/mL) IL-17A Sandwich 5589 – 7.67 (pg/mL) 3912 (pg/mL) 9.97 (pg/mL) 6.21 (pg/mL) IL-23 Sandwich 10000 – 13.72 (pg/mL) 6230 (pg/mL) 57.3 (pg/mL) 3.48 (pg/mL) IL-23 Sandwich 10000 – 13.72 (pg/mL) 6230 (pg/mL) 57.3 (pg/mL) 3.48 (pg/mL) TNFα Sandwich 661 – 0.91 (pg/mL) 462 (pg/mL) 1.18 (pg/mL) 0.10 (pg/mL) TNFα Sandwich 661 – 0.91 (pg/mL) 462 (pg/mL) 1.18 (pg/mL) 0.10 (pg/mL) TNFβ Sandwich 444 – 0.61 (pg/mL) 310 (pg/mL) 0.79 (pg/mL) 0.57 (pg/mL) TNFβ Sandwich 444 – 0.61 (pg/mL) 310 (pg/mL) 0.79 (pg/mL) 0.57 (pg/mL) Kit Components Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization. Reagents Include: Calibrator: Lyophilized, recombinant antigens in a buffered protein base Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives Streptavidin HRP (1X) : Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase Wash Buffer (20X) : Liquid, 50 mL/vial of a concentrated solution of buffered surfactant Plate Seals (3) : Adhesive strips