AffiELISA® Multiplex ELISA Kit For Human Chemokine (4-Plex)

Specifications

Description

Product Overview The Multiplex Human Chemokine (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IL-8, IP-10, MCP-1, Rantes) . Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results. Using only 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 2.25 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample. Assay Ranges Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection IL-8 Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 2.70 (pg/mL) IP-10 Sandwich 2984 – 4.09 (pg/mL) 700 (pg/mL) 5.3 (pg/mL) 4.03 (pg/mL) MCP-1 Sandwich 2000 – 2.74 (pg/mL) 467 (pg/mL) 3.6 (pg/mL) 2.60 (pg/mL) RANTES Sandwich 6703 – 9.19 (pg/mL) 4692 (pg/mL) 12.0 (pg/mL) 6.59 (pg/mL) Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection IL-8 Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 2.70 (pg/mL) IP-10 Sandwich 2984 – 4.09 (pg/mL) 700 (pg/mL) 5.3 (pg/mL) 4.03 (pg/mL) MCP-1 Sandwich 2000 – 2.74 (pg/mL) 467 (pg/mL) 3.6 (pg/mL) 2.60 (pg/mL) RANTES Sandwich 6703 – 9.19 (pg/mL) 4692 (pg/mL) 12.0 (pg/mL) 6.59 (pg/mL) IL-8 Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 2.70 (pg/mL) IL-8 Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 2.70 (pg/mL) IP-10 Sandwich 2984 – 4.09 (pg/mL) 700 (pg/mL) 5.3 (pg/mL) 4.03 (pg/mL) IP-10 Sandwich 2984 – 4.09 (pg/mL) 700 (pg/mL) 5.3 (pg/mL) 4.03 (pg/mL) MCP-1 Sandwich 2000 – 2.74 (pg/mL) 467 (pg/mL) 3.6 (pg/mL) 2.60 (pg/mL) MCP-1 Sandwich 2000 – 2.74 (pg/mL) 467 (pg/mL) 3.6 (pg/mL) 2.60 (pg/mL) RANTES Sandwich 6703 – 9.19 (pg/mL) 4692 (pg/mL) 12.0 (pg/mL) 6.59 (pg/mL) RANTES Sandwich 6703 – 9.19 (pg/mL) 4692 (pg/mL) 12.0 (pg/mL) 6.59 (pg/mL) Kit Components Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization. Reagents Include: Calibrator: Lyophilized, recombinant antigens in a buffered protein base Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives Streptavidin HRP (1X) : Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase Wash Buffer (20X) : Liquid, 50 mL/vial of a concentrated solution of buffered surfactant Plate Seals (3) : Adhesive strips