AffiELISA® Multiplex ELISA Kit For Human Angiogenesis (9-plex)

Specifications

Description

Product Overview The Multiplex Human Angiogenesis (9-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of nine biomarkers or analytes. Using only 25 µl of sample per well, up to 80 samples can be assayed for all nine markers in the panel within 2.25 hours. The Multiplex Human Angiogenesis (9-plex) provides researchers an easy to use and cost effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing nine distinct capture antibodies in a defined array to the bottom of each well. Our high quality reagents help ensure the accuracy of your results. Assay Ranges Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Ang-2 Sandwich 10,000 – 13.72 (pg/mL) 7000 (pg/mL) 17.8 (pg/mL) 3.90 (pg/mL) FGF Sandwich 5000 – 6.86 (pg/mL) 3500 (pg/mL) 8.9 (pg/mL) 1.27 (pg/mL) HGF Sandwich 14000 – 19.20 (pg/mL) 9800 (pg/mL) 25 (pg/mL) 7.20 (pg/mL) IL-8 Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 2.70 (pg/mL) PDGF Sandwich 4000 – 5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 2.20 (pg/mL) TIMP-1 Sandwich 12697 – 17.42 (pg/mL) 8888 (pg/mL) 22.6 (pg/mL) 12.95 (pg/mL) TIMP2 Sandwich 20000 – 27.43 (pg/mL) 14000 (pg/mL) 35.7 (pg/mL) 15.90 (pg/mL) TNF-α Sandwich 6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) VEGF Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 2.70 (pg/mL) Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Ang-2 Sandwich 10,000 – 13.72 (pg/mL) 7000 (pg/mL) 17.8 (pg/mL) 3.90 (pg/mL) FGF Sandwich 5000 – 6.86 (pg/mL) 3500 (pg/mL) 8.9 (pg/mL) 1.27 (pg/mL) HGF Sandwich 14000 – 19.20 (pg/mL) 9800 (pg/mL) 25 (pg/mL) 7.20 (pg/mL) IL-8 Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 2.70 (pg/mL) PDGF Sandwich 4000 – 5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 2.20 (pg/mL) TIMP-1 Sandwich 12697 – 17.42 (pg/mL) 8888 (pg/mL) 22.6 (pg/mL) 12.95 (pg/mL) TIMP2 Sandwich 20000 – 27.43 (pg/mL) 14000 (pg/mL) 35.7 (pg/mL) 15.90 (pg/mL) TNF-α Sandwich 6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) VEGF Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 2.70 (pg/mL) Ang-2 Sandwich 10,000 – 13.72 (pg/mL) 7000 (pg/mL) 17.8 (pg/mL) 3.90 (pg/mL) Ang-2 Sandwich 10,000 – 13.72 (pg/mL) 7000 (pg/mL) 17.8 (pg/mL) 3.90 (pg/mL) FGF Sandwich 5000 – 6.86 (pg/mL) 3500 (pg/mL) 8.9 (pg/mL) 1.27 (pg/mL) FGF Sandwich 5000 – 6.86 (pg/mL) 3500 (pg/mL) 8.9 (pg/mL) 1.27 (pg/mL) HGF Sandwich 14000 – 19.20 (pg/mL) 9800 (pg/mL) 25 (pg/mL) 7.20 (pg/mL) HGF Sandwich 14000 – 19.20 (pg/mL) 9800 (pg/mL) 25 (pg/mL) 7.20 (pg/mL) IL-8 Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 2.70 (pg/mL) IL-8 Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 2.70 (pg/mL) PDGF Sandwich 4000 – 5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 2.20 (pg/mL) PDGF Sandwich 4000 – 5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 2.20 (pg/mL) TIMP-1 Sandwich 12697 – 17.42 (pg/mL) 8888 (pg/mL) 22.6 (pg/mL) 12.95 (pg/mL) TIMP-1 Sandwich 12697 – 17.42 (pg/mL) 8888 (pg/mL) 22.6 (pg/mL) 12.95 (pg/mL) TIMP2 Sandwich 20000 – 27.43 (pg/mL) 14000 (pg/mL) 35.7 (pg/mL) 15.90 (pg/mL) TIMP2 Sandwich 20000 – 27.43 (pg/mL) 14000 (pg/mL) 35.7 (pg/mL) 15.90 (pg/mL) TNF-α Sandwich 6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) TNF-α Sandwich 6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) VEGF Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 2.70 (pg/mL) VEGF Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 2.70 (pg/mL) Kit Components Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization. Reagents Include: Calibrator: Lyophilized, recombinant antigens in a buffered protein base Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives Streptavidin HRP (1X) : Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase Wash Buffer (20X) : Liquid, 50 mL/vial of a concentrated solution of buffered surfactant Plate Seals (3) : Adhesive strips