AffiELISA® Human High Sensitivity (4-plex) Sample Demo Kit

Specifications

Description

Product Overview Human High Sensitivity (4-plex) Sample Kit is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 4 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 4 markers in the panel within 2.25 hours. The Human High Sensitivity (4-plex) Sample Kit provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Multiplex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results. *For a limited time, we are offering this kit on trial basis for only $99 per kit. Limit to 1 kit per lab. Claim yours today by contacting us at support@bosterbio.com for details. Assay Ranges Calibrator Range Units IL-4 2000-2.74 pg/ml IL-6 3000-4.11 pg/ml IL-10 3000-4.11 pg/ml IFN-γ 8000-10.97 pg/ml Calibrator Range Units IL-4 2000-2.74 pg/ml IL-6 3000-4.11 pg/ml IL-10 3000-4.11 pg/ml IFN-γ 8000-10.97 pg/ml Calibrator Range Units Calibrator Range Units IL-4 2000-2.74 pg/ml IL-4 2000-2.74 pg/ml IL-6 3000-4.11 pg/ml IL-6 3000-4.11 pg/ml IL-10 3000-4.11 pg/ml IL-10 3000-4.11 pg/ml IFN-γ 8000-10.97 pg/ml IFN-γ 8000-10.97 pg/ml Kit Components Each kit contains a 96-well plate (not strip well), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip well format please contact us for customization. Reagents Include: Calibrator: Lyophilized, recombinant antigens in a buffered protein base Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives Streptavidin HRP (1X) : Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase Wash Buffer (20X) : Liquid, 50 mL/vial of a concentrated solution of buffered surfactant Plate Seals (3) : Adhesive strips