AffiASSAY® Uricase Activity Assay Kit (Fluorometric)

Specifications

Description

Uricase (factor-independent urate hydroxylase, urate oxidase (UO); EC 1.7.3.3) is an enzyme involved in the uric acid metabolism. Uric acid is the end product of purine metabolism, and high levels of uric acid in blood causes gout. Uricase is present in a wide range of mammals but absent from human beings. It has been widely used for the measurement of uric acid concentrations in biological samples and recombinant uricase (rasburicase) has been used as a drug for the prevention of tumor lysis syndrome. BioVision’s Uricase Activity Assay Kit provides a quick and easy method for the measurement of uricase activity in a wide variety of samples. In this assay, uricase oxidizes uric acid forming a product that reacts with the probe, thus generating a fluorescence signal (Ex/Em= 535/587nm). The generated fluorescence signal is directly proportional to the amount of active uricase present in the sample. This kit provides a sensitive and high throughput adaptable assay and can measure uricase activity in biological samples as low as 10 µU.