AffiASSAY® Total Phosphatidic Acid Assay Kit (Fluorometric)

AffiASSAY® Total Phosphatidic Acid Assay Kit (Fluorometric)

Cat#: AFG-CBL-106
Size: 100 Assays
Price:$849.60
1
Product Details

Specifications

Category: Business & Industrial > Science & Laboratory
Brand: affiassay

Description

Our Total Phosphatidic Acid Assay Kit measures total phosphatidic acid content (PA and LPA) in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidic Acid levels in unknown sampled are determined based on the provided phosphatidic acid standard curve.

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