AffiASSAY® Plasminogen (PLG) Assay Kit (Colorimetric)

Specifications

Description

Plasminogen (PLG) is the inactive precursor of plasmin, a serine protease that degrades many blood plasma proteins including fibrin clots. PLG is processed to plasmin by endogenous activators including tissue plasminogen activator, tPA or urokinase plasminogen activators (uPA) or exogenous activators such as streptokinase. PLG is synthesized in the liver and is mainly present in plasma at a stable concentration of 200 µg/ml. Plasmin play a key role in the degradation of fibrin clots and several constituents of the extracellular matrix. PLG is a central component of the fibrinolytic system and could be a vital contributor in various diseases. Hereditary plasminogen deficiency is a predisposing risk factor for thromboembolic disease whereas acquired PLG deficiency is often seen in severe liver diseases, acute disseminated intravascular coagulation etc. PLG activators have been used in therapeutics and are also known as “clot buster”. BioVision’s Plasminogen Assay Kit is a simple, plate based assay for measuring plasminogen levels in biological samples including plasma. In this assay, excess streptokinase is first mixed with plasminogen to form plasminogen-streptokinase complex. The complex then cleaves a specific, synthetic plasmin substrate releasing pNA. The released pNA is quantified by measuring absorbance at 405 nm. The colorimetric signal is directly proportional to the amount of plasminogen in samples. The assay is specific and high-throughput adaptable. The kit can detect as low as 0.2 µg/ml of plasminogen in Samples.