AffiASSAY® Ornithine Assay Kit (Fluorometric)

Specifications

Description

Ornithine is a non-standard, non-proteogenic amino acid. Ornithine is converted to putrescine via ornithine decarboxylase, which is the first step in the synthesis of polyamines. Additionally, Ornithine is one of the main metabolites that participate in the urea cycle in order to excrete excess nitrogen in the form of urea. In cells, Arginase hydrolyzes Arginine producing Ornithine and Urea (cytosol). Then, ornithine enters the mitochondria, reacts with carbamoyl phosphate forming Citrulline. Ornithine has therapeutics uses: it is administered to patients suffering brain complications caused by hepatic encephalopathy. Additionally, early research has suggested Ornithine may improve performance in some professional athletes. BioVision’s Ornithine Assay Kit provides a rapid, specific, and easy method for the measurement of total ornithine concentrations in a wide variety of biological samples. In this enzyme-based assay, ornithine is converted into a series of intermediates, which will further react with a probe producing a stable fluorometric signal (Ex/Em = 535/587nm). The kit is simple to use, sensitive and high-throughput adaptable and can detect as low as 50 pmol/well of ornithine in biological samples.