AffiASSAY® Magnesium Colorimetric Assay Kit

Specifications

Description

Magnesium is the 11th most abundant element by mass in the human body. mg²ᶧ is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. mg²ᶧ is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. mg²ᶧ compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia. The BioVision Magnesium Assay Kit provides a simple sensitive means of quantitating magnesium in a variety of biological samples. The kit takes advantage of the specific requirement of glycerol kinase for mg²ᶧ. An enzyme linked reaction leads to formation of an intensely colored (λmax = 450nm) product whose formation is proportional to mg²ᶧ concentration. The linear range of the assay is 2-15 nmoles with detection sensitivity~ 40 µM.