
Description
For quantitative colorimetric determination of isocitrate and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 20 to 5000 µM for 10 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.). Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 20 µM.
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