AffiASSAY® Inorganic Polyphosphate Assay Kit (Fluorometric)

Specifications

Description

Inorganic polyphosphate (Poly P) is a linear polymer of hundreds of orthophosphate (Pi) residues linked by high-energy, phosphoanhydride bonds. Poly P is ubiquitous and can be found in all living organisms from bacteria to mammals. Poly P has many important cellular functions. In microorganisms, Poly P plays an important role in virulence, biofilm formation, motility, quorum sensing, stress response and survival during nutrient deficiency. In mammals, it is important for blood coagulation, calcium precipitation, immune response, apoptosis, signal transduction and mitochondrial metabolism. Poly P also plays an important role in cancer cell proliferation. The traditional assay for Poly P quantification uses radioisotope methods, which are not convenient to perform. BioVision’s Inorganic Polyphosphate Assay Kit is a simple assay to quantify the amount of Poly P in various biological samples. The assay uses a fluorescent dye that forms a complex with Poly P present in the samples and the fluorsecent complex is measured at Ex/Em = 415/550 nm. The fluorescent signal is proportional to the Poly P concentration in the samples. BioVision’s Inorganic Polyphosphate Assay Kit is rapid, sensitive and a convenient tool for detecting Poly P. It can detect as low as 50 pmole under the assay conditions.