AffiASSAY® Hypoxanthine Phosphoribosyl Transferase Activity Assay Kit (Fluorometric)

Specifications

Description

Hypoxanthine Phosphoribosyl Transferase or HPRT (EC 2.4.2.8) plays an important role in the generation of purine nucleotides through the purine salvage pathway and is present in prokaryotes and eukaryotes. The enzyme converts guanine to guanosine monophosphate and hypoxanthine to inosine monophosphate by transferring a phosphoribosyl group from phosphoribosyl pyrophosphate (PRPP) to the purine molecule, and releases an inorganic pyrophosphate molecule in the process. Mutations in HPRT lead to developmental disorders such as Lesch Nyhan Disease (LND), characterized by neurological and behavioral abnormalities. Its deficiency also leads to defective basal ganglia expression of the neurotransmitter dopamine and aberrant neuronal function. This causes dysregulation of multiple dopamine- related developmental functions and cellular signaling defects. BioVision’s HPRT Activity Assay Kit is a simple one-step plate based assay kit for the measurement of HPRT activity in biological samples. HPRT catalyzes the conversion of hypoxanthine to inosine monophosphate (IMP). IMP then undergoes a series of enzymatic reactions to convert a non-fluorescent probe to a fluorescent product. The signal, which is proportional to the generated product can be read in kinetic mode at Ex/Em 535/587 nm. The assay can detect as low as 2 mU HPRT.