AffiASSAY® Glyoxalase I Activity Assay Kit (Colorimetric)

Specifications

Description

Glyoxalase I (GloI, lactoylglutathione lyase, EC 4.4.1.5) is a cytosolic enzyme that participates in the Glyoxalase system. The glyoxalase system is a metabolic pathway that detoxifies α-ketoaldehydes such as methylglyoxal to D-lactic acid, via the intermediate S-D-lactoyl-glutathione (SLG). Specifically, GloI catalyzes the formation of SLG from methylglyoxal and reduced Glutathione. It is overexpressed in several human cancers such as colon, breast, prostate and melanoma. GloI also enhances resistance to antitumor agent-induced apoptosis in human leukemia cells. In addition, recent findings have linked GloI to numerous anxiety-like behaviors including psychiatric diseases (depression, schizophrenia and autism) and pain. BioVision’s Glyoxalase I Activity Assay Kit utilizes the ability of an active GloI to catalyze the formation of SLG by using two GloI substrates. The formation of the SLG can be tracked using a microplate reader at OD 240 nm. Our assay kit is simple, sensitive and can detect as low as 0.05 mU of Glo I activity in biological samples.