AffiASSAY® Glycerophosphorylcholine Assay Kit (Colorimetric/Fluorometric)

Specifications

Description

Glycerophosphorylcholine (GPC), also referred to as alpha-GPC or choline alfoscerate, is a small metabolite and potential dementia treatment. GPC is a breakdown product of phosphatidylcholine, and dysregulation of phospholipid metabolism can alter lipid and metabolite concentrations, leading to health problems such as fatty liver, Gaucher’s disease, and Tay-Sachs disease. GPC is also component of seminal fluid and breast milk, and a precursor of the neurotransmitter, acetylcholine. As GPC can efficiently cross the blood-brain barrier, it has been hypothesized as a treatment for Alzheimer’s disease and other forms of dementia. In such cases, GPC serves as a biomarker for lipid membrane decomposition. BioVision’s Glycerophosphorylcholine Assay Kit allows a quick and sensitive means to determine GPC concentration in a biological sample. The assay is robust and specific, with a GPC enzyme mix converting GPC in the sample into an intermediate that is then utilized to quantitatively generate signal using the GPC Developer mix and GPC Probe. The included Sample Clean-Up Mix allows the user to avoid high background from choline, which is present at high levels in many biological fluids. The assay is rapid, simple, high throughput compatible, and can detect GPC concentrations as low as 0.1 µM in tissue lysates and other samples such as breast milk and seminal plasma.