AffiASSAY® DNA Quantification Assay Kit (Fluorometric)

Specifications

Description

DNA quantification is essential for a variety of applications, such as real-time PCR (rtPCR), qPCR, Next Generation Sequencing (NGS), etc. The measurement of DNA using analytical instruments (i.e. “nanodrop”) is often limited by the presence/contamination of other macromolecules like protein and RNAs found in biological samples. However, the development of highly specific probes that bind to DNA allows the measurement of this macromolecule using a fluorimeter. The biggest advantages of this approach is protein, RNA and single-stranded DNA (ssDNA) are not detected. BioVision’s DNA Quantification Assay Kit provides a quick, specific, and easy method for the measurement of DNA concentrations in a wide variety of samples. In this assay, DNA specifically reacts with a probe producing a stable fluorescence signal (Ex/Em = 492/528 nm). The kit is simple to perform, specific, sensitive and high-throughput adaptable with a wide detection range (0.4-150 ng DNA) and a limit of detection of 4 pg/µl dsDNA in samples.